噬菌体展示技术(英語:Phage display),将编码所需产物的外源 DNA 片段插入噬菌体基因组,并使之与噬菌体衣壳蛋白编码基因或其他结构基因相融合,然后用该重组噬菌体侵染宿主细菌,复制形成大量带有杂合衣壳蛋白(或其他结构蛋白)的噬菌体颗粒,从而捕获 cDNA 表达文库中与“诱饵”相互作用的蛋白质[1]。
噬菌體展示週期。 1) 病毒外殼蛋白+要被進化的基因(通常是抗體片段)的融合蛋白在噬菌體中表達。 2) the library of phage are washed over an immobilised target. 3) the remaining high-affinity binders are used to infect bacteria. 4) the genes encoding the high-affinity binders are isolated. 5) those genes may have random mutations introduced and used to perform another round of evolution. The selection and amplification steps can be performed multiple times at greater stringency to isolate higher-affinity binders.