The following highways are numbered 401:
Canada
- British Columbia Highway 401 (former)
- Manitoba Provincial Road 401
Ontario Highway 401
Costa Rica
Japan
Norway
Thailand
United States
U.S. Route 401- Florida:
Georgia State Route 401 (unsigned designation for Interstate 75)
Kentucky Route 401
Nevada State Route 401- New York:
North Carolina Highway 401 (former)
Pennsylvania Route 401
Puerto Rico Highway 401
Rhode Island Route 401
Virginia State Route 401
Washington State Route 401
| Preceded by 400 | Lists of highways 401 | Succeeded by 402 |