dnaC is a loading factor that complexes with the C-terminus of helicase dnaB and inhibits it from unwinding the dsDNA at a replication fork.[1] A dnaB and dnaC associate near the dnaA bound origin for each of the ssDNA.[1] One dnaB-dnaC complex is oriented in the opposite direction to the other dnaB-dnaC complex due to the antiparallel nature of DNA. Because they are oriented in opposite directions, one dnaB-dnaC complex will complex with dnaA from the N-terminus of dnaB whereas the other dnaB-dnaC complex will complex with dnaA from the dnaC. After the assembly of dnaG onto the N-terminus of dnaB, dnaC is released and dnaB will be allowed to begin unwinding dsDNA to make room for DNA polymerase III to begin synthesizing the daughter strands.[1]
| DNA replication gene dnaC | |||||||
|---|---|---|---|---|---|---|---|
| Identifiers | |||||||
| Organism | |||||||
| Symbol | dnaC | ||||||
| Entrez | 948864 | ||||||
| RefSeq (Prot) | NP_418781.1 | ||||||
| UniProt | P0AEF0 | ||||||
| Other data | |||||||
| EC number | 2.7.7.7 | ||||||
| Chromosome | genome: 4.6 - 4.6 Mb | ||||||
| |||||||
This interaction of dnaC with dnaB requires the hydrolysis of ATP.[2]
References
External links
- DnaC+protein,+E+coli at the U.S. National Library of Medicine Medical Subject Headings (MeSH)